This combination is then incubated at 55°C for a single hour. Then the sample is phenol-extracted when with phenol/chloroform/isoamyl alcohol Answer and centrifuged. The aqueous layer is eliminated to a fresh new microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a 2nd time. After the pellet is https://www.pinterest.com/pin/993958580252595370/